Fermentative Production of Lysine by Corynebacterium glutamicum from Different Carbon Sources

fermentative production of lysine by corynebacterium glutamicum from different carbon sources

production of lysine by corynebacterium glutamicum (ptcc 1532) from different agricultural by-products (molasses and pulpy waste date) was compared to glucose as raw materials. for this purpose, ammonium sulphate was selected as a constant nitrogen source. the effect of different nitrogen sources was also investigated with glucose as a constant carbon source. the production of l-lysine was exam...

Enhanced production of L-lysine from Corynebacterium glutamicum by mutagenesis

Modeling the Fermentative Production of L-glutamic Acid by Corynebacterium Glutamicum in a Batch Bioreactor

The fermentation kinetics of L-glutamic acid by Corynebacterium glutamicum was studied in a batch bioreactor. Mathematical model using the logistic equation for growth, Leudeking-Piret kinetic equation for product formation and Leudeking-Piret like equation for substrate consumption was proposed. Based on the analysis of experimental data followed by computer simulation, the model seemed to pro...

Lysine uptake and exchange in Corynebacterium glutamicum.

Resting cells of Corynebacterium glutamicum (ATCC 13032) accumulate [14C]lysine by a transport system with a relatively high affinity (10 microMs) and a low maximum velocity (0.15 nmol/min per mg [dry weight]). Uptake of lysine was not inhibited by uncouplers or by ionophores affecting the ion gradients and the energetic state of the cell. Analysis of intracellular amino acid concentrations dur...

Amplified expression of fructose 1,6-bisphosphatase in Corynebacterium glutamicum increases in vivo flux through the pentose phosphate pathway and lysine production on different carbon sources.

The overexpression of fructose 1,6-bisphosphatase (FBPase) in Corynebacterium glutamicum leads to significant improvement of lysine production on different sugars. Amplified expression of FBPase via the promoter of the gene encoding elongation factor TU (EFTU) increased the lysine yield in the feedback-deregulated lysine-producing strain C. glutamicum lysCfbr by 40% on glucose and 30% on fructo...

Structural basis for cytokinin production by LOG from Corynebacterium glutamicum

"Lonely guy" (LOG) has been identified as a cytokinin-producing enzyme in plants and plant-interacting fungi. The gene product of Cg2612 from the soil-dwelling bacterium Corynebacterium glutamicum was annotated as an LDC. However, the facts that C. glutamicum lacks an LDC and Cg2612 has high amino acid similarity with LOG proteins suggest that Cg2612 is possibly an LOG protein. To investigate t...